rna in situ hybridization  (Advanced Cell Diagnostics Inc)

Journal: Cell stem cell

Article Title: Single-Cell Analysis of the Liver Epithelium Reveals Dynamic Heterogeneity and an Essential Role for YAP in Homeostasis and Regeneration

doi: 10.1016/j.stem.2019.04.004

Figure Lengend Snippet: (A) Cyr61-Klf6 and Cyr61-Hes1 RNA-ISH combined with IF stain for pan-Cytokeratin (pCK) of mouse liver sections. Arrows indicate BECs co-expressing Cyr61 and Klf6 RNA, and arrowheads RNA-negative molecules.

Article Snippet: RNA In Situ Hybridization RNAscope Multiplex Fluorescent Detection kit v2 (ACDbio) in combination with TSA Fluorescein and Cyanine3 Plus Evaluation kits (Perkin Elmer) were used for RNA in situ hybridization according to manufacturer’s instructions.

Techniques: Staining, Expressing

Journal: Cell stem cell

Article Title: Single-Cell Analysis of the Liver Epithelium Reveals Dynamic Heterogeneity and an Essential Role for YAP in Homeostasis and Regeneration

doi: 10.1016/j.stem.2019.04.004

Figure Lengend Snippet: (A) Heatmap of scRNA-seq analysis of hepatocytes isolated from mice either fed a regular (Control) or DDC diet for 1 week, each with cells ordered according to their predicted relative position from the central (left) to the portal vein (right), using landmark genes (exemplary Alb, Cyp2f2 and Cyp2e1 in blue). Colors denote relative expression for shown candidate genes (highlighted: biliary markers (green) YAP targets from BECs (black), RNA-ISH analyzed genes (red).

Article Snippet: RNA In Situ Hybridization RNAscope Multiplex Fluorescent Detection kit v2 (ACDbio) in combination with TSA Fluorescein and Cyanine3 Plus Evaluation kits (Perkin Elmer) were used for RNA in situ hybridization according to manufacturer’s instructions.

Techniques: Isolation, Control, Expressing